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mouse anti-synapsin 1 106001  (Synaptic Systems)


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    Synaptic Systems mouse anti-synapsin 1 106001
    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, <t>Syn1,</t> Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.
    Mouse Anti Synapsin 1 106001, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-synapsin+1+106001/bio_rxiv__399717-52-42-45?v=Synaptic+Systems
    Average 90 stars, based on 1 article reviews
    mouse anti-synapsin 1 106001 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Evidence of Müller glia conversion into retina ganglion cells using Neurogenin2"

    Article Title: Evidence of Müller glia conversion into retina ganglion cells using Neurogenin2

    Journal: bioRxiv

    doi: 10.1101/399717

    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, Syn1, Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.
    Figure Legend Snippet: (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, Syn1, Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.

    Techniques Used: Expressing

    (A-L) Immunolabeling of the RGC proteins RBPMS (red, A-C), β III -TUBULIN (green, D-F), PARV (red, G-I) and SYN1 (green, J-L) in MGC converted into iNs at 15 days post-nucleofection with either Neurog2-I-GFP (green, A-C and G-I) or Neurog2-I-DsRed (red, D-F and J-L). Scale bars: 20 µm.
    Figure Legend Snippet: (A-L) Immunolabeling of the RGC proteins RBPMS (red, A-C), β III -TUBULIN (green, D-F), PARV (red, G-I) and SYN1 (green, J-L) in MGC converted into iNs at 15 days post-nucleofection with either Neurog2-I-GFP (green, A-C and G-I) or Neurog2-I-DsRed (red, D-F and J-L). Scale bars: 20 µm.

    Techniques Used: Immunolabeling



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    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, <t>Syn1,</t> Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.
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    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, <t>Syn1,</t> Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.
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    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, <t>Syn1,</t> Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.
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    Image Search Results


    (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, Syn1, Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.

    Journal: bioRxiv

    Article Title: Evidence of Müller glia conversion into retina ganglion cells using Neurogenin2

    doi: 10.1101/399717

    Figure Lengend Snippet: (A) Expression levels of selected genes used to identify different classes of retina neurons using scRNAseq (see also Fig S4). Dot sizes and colors (green-low to red-high) represent the percentage of cells expressing the gene and its average scaled expression, respectively. Observe that the expression of Slc17a6 and Pou4f1 are specific for a high percentage of cells assigned as RGCs. (B-C) Graphics showing the relative expression levels (log 10 ) of genes enriched in Müller glia (Rlbp1, Glul), photoreceptors (Rho, Rcvrn, Pde6g), horizontal (Prox1, Lhx1), bipolar (Vsx2), amacrine (Slc32a1, Chat, Th) and retina ganglion cells (Pou4f1, Slc17a6, Calb2, Rbfox3, Syn1, Pvalb, Rbpms), as shown in A, in MGC cultures nucleofected with either Neurog2 (B) or Ascl1 (C). White and black bars indicate that MGC were expanded in the absence or presence of EGF/FGF2, respectively, prior to the expression of Neurog2 or Ascl1.

    Article Snippet: Primary antibodies and respective dilutions were: chicken anti-Green Fluorescent Protein (Aves Labs, cat#GFP-1020, 1:1000), rabbit anti-Red Fluorescent Protein (Rockland, cat#600- 401-379, 1:1000), mouse anti-microtubule associated protein (Sigma, cat#M1406, 1:500), mouse anti-β III -TUBULIN (TUBB3; Biolegend, cat#MMS-435P, 1:1000), rabbit anti-RBPMS (PhosphoSolutions, cat#1830; 1:100), mouse anti-SYNAPSIN 1 (Synaptic Systems, cat#106001, 1:2000), mouse anti-PARVALBUMIN (SIGMA, cat#p3088, 1:1000), mouse anti-CRALBP (ABCAM, cat# ab15051, 1:500), rabbit anti-GFAP (DakoCytomation, cat#z0334, 1:4000) rabbit anti-SOX2 (ABCAM, cat# ab97959, 1:500), rat anti-BrdU (ABCAM, cat# ab6326 1:500), rabbit anti-phospho-histone 3 (Millipore, cat#06-570, 1:1000), mouse anti-PAX6 (Millipore, cat#MAB5552, 1:500) and mouse anti-NESTIN (Millipore, cat#mab353, 1:200 millipore).

    Techniques: Expressing

    (A-L) Immunolabeling of the RGC proteins RBPMS (red, A-C), β III -TUBULIN (green, D-F), PARV (red, G-I) and SYN1 (green, J-L) in MGC converted into iNs at 15 days post-nucleofection with either Neurog2-I-GFP (green, A-C and G-I) or Neurog2-I-DsRed (red, D-F and J-L). Scale bars: 20 µm.

    Journal: bioRxiv

    Article Title: Evidence of Müller glia conversion into retina ganglion cells using Neurogenin2

    doi: 10.1101/399717

    Figure Lengend Snippet: (A-L) Immunolabeling of the RGC proteins RBPMS (red, A-C), β III -TUBULIN (green, D-F), PARV (red, G-I) and SYN1 (green, J-L) in MGC converted into iNs at 15 days post-nucleofection with either Neurog2-I-GFP (green, A-C and G-I) or Neurog2-I-DsRed (red, D-F and J-L). Scale bars: 20 µm.

    Article Snippet: Primary antibodies and respective dilutions were: chicken anti-Green Fluorescent Protein (Aves Labs, cat#GFP-1020, 1:1000), rabbit anti-Red Fluorescent Protein (Rockland, cat#600- 401-379, 1:1000), mouse anti-microtubule associated protein (Sigma, cat#M1406, 1:500), mouse anti-β III -TUBULIN (TUBB3; Biolegend, cat#MMS-435P, 1:1000), rabbit anti-RBPMS (PhosphoSolutions, cat#1830; 1:100), mouse anti-SYNAPSIN 1 (Synaptic Systems, cat#106001, 1:2000), mouse anti-PARVALBUMIN (SIGMA, cat#p3088, 1:1000), mouse anti-CRALBP (ABCAM, cat# ab15051, 1:500), rabbit anti-GFAP (DakoCytomation, cat#z0334, 1:4000) rabbit anti-SOX2 (ABCAM, cat# ab97959, 1:500), rat anti-BrdU (ABCAM, cat# ab6326 1:500), rabbit anti-phospho-histone 3 (Millipore, cat#06-570, 1:1000), mouse anti-PAX6 (Millipore, cat#MAB5552, 1:500) and mouse anti-NESTIN (Millipore, cat#mab353, 1:200 millipore).

    Techniques: Immunolabeling